Alert	Value	Detail
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Estimated Number of Cells

12,500

Post-Normalization Mean Reads per Cell

19,620

1,100

Pre-Normalization Number of Reads: Total number of single-end reads that were sequenced.
Post-Normalization Number of Reads: Number of single-end reads after normalizing for depth among multiple libraries.

Pre-Normalization Number of Reads	270,518,548
Post-Normalization Number of Reads	245,257,381

mouse32 Dnmt3a WT Fraction of Reads Kept: The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
mouse31 Dnmt3a KO Fraction of Reads Kept: The fraction of reads kept for this input sample, after normalizing depth across samples to reduce batch effects.
mouse32 Dnmt3a WT Pre-Normalization Total Reads per Cell: The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
mouse31 Dnmt3a KO Pre-Normalization Total Reads per Cell: The mean number of total sequencing reads per cell in this input sample, prior to depth normalization.
mouse32 Dnmt3a WT Pre-Normalization Confidently Mapped Barcoded Reads per Cell: The mean number of confidently mapped, barcoded reads per cell in this input sample, prior to depth normalization.
mouse31 Dnmt3a KO Pre-Normalization Confidently Mapped Barcoded Reads per Cell: The mean number of confidently mapped, barcoded reads per cell in this input sample, prior to depth normalization.

mouse32 Dnmt3a WT Fraction of Reads Kept	81.4%
mouse31 Dnmt3a KO Fraction of Reads Kept	100.0%
mouse32 Dnmt3a WT Pre-Normalization Total Reads per Cell	25,276
mouse31 Dnmt3a KO Pre-Normalization Total Reads per Cell	18,896
mouse32 Dnmt3a WT Pre-Normalization Confidently Mapped Barcoded Reads per Cell	14,351
mouse31 Dnmt3a KO Pre-Normalization Confidently Mapped Barcoded Reads per Cell	11,684

Estimated Number of Cells: The total number of barcodes associated with cell-containing partitions, estimated from the barcode count distribution.
Fraction Reads in Cells: The fraction of barcoded, confidently mapped reads with cell-associated barcodes.
Pre-Normalization Mean Reads per Cell: The total number of sequenced reads divided by the number of barcodes associated with cell-containing partitions.
Post-Normalization Mean Reads per Cell: The total number of (depth-normalized) reads divided by the number of barcodes associated with cell-containing partitions.
Median Genes per Cell: The median number of genes detected per cell-associated barcode. Detection is defined as the presence of at least 1 UMI count.
Median UMI Counts per Cell: The median number of UMI counts per cell-associated barcode.

Estimated Number of Cells	12,500
Fraction Reads in Cells	87.8%
Pre-Normalization Mean Reads per Cell	21,641
Post-Normalization Mean Reads per Cell	19,620
Median Genes per Cell	1,100
Median UMI Counts per Cell	3,720

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